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弱精子症不育的分子病因研究(我们的成果)

王增军
王增军主任医师 教授江苏省人民医院 泌尿外科

J Assist Reprod Genet. 2010 Dec;27(12):719-24. Epub 2010 Sep 1.

Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.

Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. 江苏省人民医院泌尿外科王增军

Abstract

PURPOSE: to analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.

METHODS: high motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility.

RESULTS: real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups.

CONCLUSION: the high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.

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发布时间:2011-01-30 18:36

王增军主任医师 教授
江苏省人民医院 泌尿外科
擅长:肾癌前列腺癌膀胱癌的诊治,男性不育性功能障碍的诊治;机器人辅助腹腔镜下复杂性泌尿生殖系统肿瘤如肾癌根治及保留肾单位肾部分切除术,保留性神经膀胱癌根治性切除原位新膀胱术,保留性神经及尿控功能前列腺癌根治术,睾丸癌腹膜后淋巴结清扫等微创治疗,尿道断裂尿道修复重建手术,泌尿生殖系统先天性畸形如肾盂输尿管连接部狭窄和小儿尿道下裂修复重建等。前列腺增生症微创治疗,男性勃起功能障碍假体植入,血精的微创治疗,男性不育(少弱精及无精子症)的诊治等。 << 收起

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